Department of Internal Medicine

Pulmonary, Critical Care and
Occupational Medicine Faculty

John Engelhardt photo

Education:
B.S., Biochemistry, Iowa State University, 1985
Ph.D., Human Genetics,
Johns Hopkins University, 1990

Fellowship:
University of Michigan

John F. Engelhardt, Ph.D.
Professor of Medicine and Anatomy and Cell Biology
Roy J. Carver Chair in Molecular Medicine
Director, Gene Therapy Center for Cystic Fibrosis and Other Genetic Diseases and Director, Animal Models Core
Interim Head of Anatomy and Cell Biology

Research in Dr. Engelhardt's laboratory focuses on the development of gene therapies for inherited and environmentally induced diseases. Included in these areas of molecular medicine are four areas of research emphasis: 1) pathogenesis and treatment of cystic fibrosis lung disease, 2) mechanisms of submucosal gland development and the identification of stem cells in the airway, 3) redox-mediated mechanisms of ischemia/reperfusion injury in the liver and heart, and 4) biology of adeno-associated virus (AAV) transduction and development of this virus as a vector for gene therapy.

Honors, Awards, and Organizations

Recent Publications

  1. Driskell, R.R., Liu, X., Luo, M., Filali, M., Zhou, W., Abbott, D., Cheng, N., Moothart, C., Sigmund, C.D., and Engelhardt, J.F. 2004. Wnt-Responsive Element Controls Lef-1 Promoter Expression During Submucosal Gland Morphogenesis. Am J Physiol Lung Cell Mol Physiol . (In Press)
  2. Liu, X., Yan, Z., Luo, M., Zak, R., Li, Z., Driskell, R.R., Huang, Y., Tran, N., and Engelhardt, J.F. 2004. Targeted correction of single-base-pair mutations with adeno-associated virus vectors under nonselective conditions. J Virol 78:4165-4175.
  3. Fan, C., Li, Q., Zhang, Y., Liu, X., Luo, M., Abbott, D., Zhou, W., and Engelhardt, J.F. 2004. IkappaBalpha and IkappaBbeta possess injury context-specific functions that uniquely influence hepatic NF-kappaB induction and inflammation. J Clin Invest 113:746-755.
  4. Liu, X., Driskell, R.R., Luo, M., Abbott, D., Filali, M., Cheng, N., Sigmund, C.D., and Engelhardt, J.F. 2004. Characterization of Lef-1 Promoter Segments that Facilitate Inductive Developmental Expression in Skin. J Invest Dermatol 123:264-274.
  6. Yan, Z., Zak, R., Zhang, Y., Ding, W., Godwin, S., Munson, K., Peluso, R., and Engelhardt, J.F. 2004. Distinct classes of proteasome-modulating agents cooperatively augment recombinant adeno-associated virus type 2 and type 5-mediated transduction from the apical surfaces of human airway epithelia. J Virol 78:2863-2874.
  7. Ding, W., Yan, Z., Zak, R., Saavedra, M., Rodman, D.M., and Engelhardt, J.F. 2003. Second-strand genome conversion of adeno-associated virus type 2 (AAV-2) and AAV-5 is not rate limiting following apical infection of polarized human airway epithelia. J Virol 77:7361-7366.
  8. Yang, J., Marden, J.J., Fan, C., Sanlioglu, S., Weiss, R.M., Ritchie, T.C., Davisson, R.L., and Engelhardt, J.F. 2003. Genetic redox preconditioning differentially modulates AP-1 and NF kappa B responses following cardiac ischemia/reperfusion injury and protects against necrosis and apoptosis. Mol Ther 7:341-353.
  9. Duan, D., Yue, Y., and Engelhardt, J.F. 2003. Consequences of DNA-dependent protein kinase catalytic subunit deficiency on recombinant adeno-associated virus genome circularization and heterodimerization in muscle tissue. J Virol 77:4751-4759.
  10. Xiong, S., She, H., Takeuchi, H., Han, B., Engelhardt, J.F., Barton, C.H., Zandi, E., Giulivi, C., and Tsukamoto, H. 2003. Signaling role of intracellular iron in NF-kappaB activation. J Biol Chem 278:17646-17654.
  11. Li, Z., Rezaei Sabet, M., Zhou, Q., Liu, X., Ding, W., Zhang, Y., Renard, J.P., and Engelhardt, J.F. 2003. Developmental capacity of ferret embryos by nuclear transfer using G0/G1-phase fetal fibroblasts. Biol Reprod 68:2297-2303.
  12. Tran, N.D., Liu, X., Yan, Z., Abbote, D., Jiang, Q., Kmiec, E.B., Sigmund, C.D., and Engelhardt, J.F. 2003. Efficiency of chimeraplast gene targeting by direct nuclear injection using a GFP recovery assay. Mol Ther 7:248-253.
  13. Fan, C., Yang, J., and Engelhardt, J.F. 2002. Temporal pattern of NFkappaB activation influences apoptotic cell fate in a stimuli-dependent fashion. J Cell Sci 115:4843-4853.
  14. Fan, C., Li, Q., Ross, D., and Engelhardt, J.F. 2003. Tyrosine phosphorylation of I kappa B alpha activates NF kappa B through a redox-regulated and c-Src-dependent mechanism following hypoxia/reoxygenation. J Biol Chem 278:2072-2080.
  15. Filali, M., Cheng, N., Abbott, D., Leontiev, V., and Engelhardt, J.F. 2002. Wnt-3A/beta-catenin signaling induces transcription from the LEF-1 promoter. J Biol Chem 277:33398-33410.
  16. Yan, Z., Zak, R., Luxton, G.W., Ritchie, T.C., Bantel-Schaal, U., and Engelhardt, J.F. 2002. Ubiquitination of both adeno-associated virus type 2 and 5 capsid proteins affects the transduction efficiency of recombinant vectors. J Virol 76:2043-2053.
  17. Liu, X., Jiang, Q., Mansfield, S.G., Puttaraju, M., Zhang, Y., Zhou, W., Cohn, J.A., Garcia-Blanco, M.A., Mitchell, L.G., and Engelhardt, J.F. 2002. Partial correction of endogenous DeltaF508 CFTR in human cystic fibrosis airway epithelia by spliceosome-mediated RNA trans-splicing. Nat Biotechnol 20:47-52.

Links of Interest

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