Department of Internal Medicine
Nephrology Faculty
John B. Stokes, M.D.
Professor
Director, Division of Nephrology
Dr. Stokes' research addresses the mechanisms whereby ions are translocated across cell membranes and how these processes are regulated. Most recently he has focused on understanding how Na is translocated across the Epithelial Na Channel, (ENaC). This ion channel is composed of three different subunits (alpha, beta, and gamma). Steroid hormones as well as several peptide hormones regulate this ion channel. Mutations of two of these subunits can produce activation of the channel and are responsible for the syndrome of hypertension and hypokalemia called Liddle Syndrome. The regulation of this ion channel is of critical importance in blood pressure regulation and Na and K homeostasis. The molecular interactions responsible for this regulation are being unraveled and structural proteins and kinases responsible for proper functioning of the channel are being studied. Methods used in Dr. Stokes' laboratory to study this channel include electrophysiology of model epithelial monolayers, heterologous expression in Xenopus oocytes, overexpression of proteins using adenovirus, structural alteration with functional analysis, immunofluorescence, genetic engineering and targeting, and biochemical analyses of second messengers. Dr. Stokes is the Director of the NIH supported O'Brien Kidney Research Center at the University of Iowa. This Center is investigating some aspects of ENaC regulation.
Dr Stokes also coordinates the University of Iowa site of a clinical trial on Nocturnal Dialysis, a NIH-sponsored study examining the effects of intensive dialysis on patients with End Stage Renal Disease. This study is designed to evaluate effects on hospitalization, quality of life, and cardiovascular complications in patients on dialysis.
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Medical School:
Temple University
Residency:
Washington University
University of Texas, Southwestern
Fellowship:
University of Texas, Southwestern |
Honors, Awards, and Organizations
- Alexander von Humboldt Prize
- Alpha Omega Alpha
- American Association for the Advancement of Science
- Diplomate, American Board of Internal Medicine
- American College of Physicians, Fellow
- American Heart Association
- Council for High Blood Pressure Research, Fellow
- Council on Kidney Disease, Established Investigator Award
- American Physiological Society
- American Society for Clinical Investigation
- American Society for Nephrology
- Association of American Physicians
- Central Society for Clinical Research
- Editorial Board: American Journal of Kidney Disease
- Editorial Board: American Journal of Physiology : Renal, Fluid, and Electrolyte Metabolism
- Editorial Board: Journal of Clinical Investigation
- International Society of Nephrology
- NIH Physiology Study Section
- Secretary-Treasurer, American Society of Nephrology, 1997-2003
- Consulting Editor, Journal of Clinical Investigation 1997-2003
- President's Medal of the American Society of Nephrology
Recent Publications
- Cao, X., Shi, P., Sigmund, R., Husted, R., Sigmund, C.D., Williamson, R., Stokes, J.B., and Yang, B.,: Mice Heterozygous for Beta-ENaC Deletion have Defective Potassium Excretion. Am. J. Physiol. Renal, (In Press), 2006.
- Olakanmi, O., Stokes, J., and Britigan, B.E.: Gallium-Inducible Transferrin-Independent Iron Acquisition is a Property of Many Cell Types: Possible Role of Alterations in the Plasma Membrane. J. Invest. Med., 53(3):143-153, 2005.
- Uc, A., Husted, R.F., Giriyappa, R.L., Britigan, B.E., and Stokes, J.B.: Hemin Induces Active Chloride Secretion in Caco-2 Cells. Am. J. Physiol. Gastrointest. Liver Physiol., 289:G202-G208, 2005.
- Click here for additional publications.
Links of Interest
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